Label Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm) Complete Your Research Human alpha-Smooth Muscle Actin PE-conjugated Antibody Cat # IC1420P Citations (14) 2018;15(5):6195-202. Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Definition / general. Oncol Lett. IC1420T 3 Citations Submit a Review Datasheet. Introduction. Human alpha-Smooth Muscle Actin Alexa Fluor 594-conjugated Antibody . As the main regulator, hypoxia-inducible factor-1 (HIF-1) was highly expressed in activated fibroblasts and increased the content of LDs. Table 2 Lineage marker antibody cocktail Full size table . Four CAF cell lines were isolated from patients with luminal breast cancer (lumBC) and classified according to the presence of -SMA protein. Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal disease that typically features a dramatic desmoplastic reaction, especially fibroblasts. Although -smooth muscle actin (-SMA) expression is the hallmark of mature myofibroblasts, its role in regulating fibroblast function remains poorly understood. The expression levels of alpha-smooth muscle actin (alpha-SMA, a marker of fibroblast activation) in fibrotic livers were significantly higher than those in control livers. Fig. BMC Cardiovasc. Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse ab254015 contains multiple trial-sized versions of anti-human and mouse antibody clones against Vimentin, alpha smooth muscle Actin, Hsp47, S100A4, specifically selected for high performance in various applications. To determine how hyperoxia changed PDGFRA + fibroblast differentiation to myofibroblasts, we stained for the myofibroblast marker alpha-smooth muscle actin (-SMA) and performed flow cytometry (Supplemental Figure 3A) (11, 16, 20, 54). Fibroblast-specific protein 1 (FSP1) was rarely expressed in SMA + PMCs of prepubertal testes, while in adult testes, majority of SMA . Research into stemness marker expression in ameloblastic tumours is lacking. PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm) Complete Your Research . 2017 . Tested in Human, Mouse, Rat, Zebrafish. Alpha-smooth muscle actin (alpha-SMA) is the actin isoform that predominates within vascular smooth-muscle cells and plays an important role in fibrogenesis (Kawasaki et al., 2008). . Purpose: The aim of this study is to investigate the differential expression of -sma-positive fibroblasts (CAFs) in nasopharyngeal carcinomas (NPCs), nasopharyngitis, metastatic tissues of NPCs and its prognostic value in NPCs. Alpha-smooth muscle actin is a 42 kDa protein and is a major component of the cytoskeletal structural network. The . IL-6 deficient (IL-6KO) mice display significantly delayed cutaneous wound closure. It reacts with tumors arising from smooth muscles and myoepithelial cells. . In this study, we used a combination of immunohistochemistry and correlative confocal scanning laser and electron microscopy to investigate the structural organization of -SMA in relation to the nucleus. Numerous biomarkers have been considered CAF markers, including but not limited to: alpha-smooth muscle actin (-SMA), fibroblast activation protein (FAP), fibroblast specific protein 1 (FSP1 . SMA is best known for its role in wound healing, where it is one of the major causes of myofibroblast . Here, we report the character and behavior of peritubular cells expressing alpha smooth muscle actin (SMA), a marker of PMCs in adult testes, in prepubertal testes procured from 5-months-old bulls. Activation of cardiac fibroblasts is a key step in development of cardiac fibrosis. Alpha-smooth muscle actin (-SMA) is the primary marker of myofibroblasts [32,37]. We have investigated the role of -SMA for myofibroblast adhesion and FA maturation. -SMA: a snapshot of CAF heterogeneity As the marker of activated fibroblasts, -SMA, a cytoskel-etal protein associated with TGF- production and highly contractile phenotype, is the first identified and most fre-quent CAF biomarker [8, 9]. This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. . -SMA: a snapshot of CAF heterogeneity As the marker of activated fibroblasts, -SMA, a cytoskeletal protein associated with TGF- production and highly contractile phenotype, is the first identified and most frequent CAF biomarker [ 8, 9 ]. Cells lung fibroblasts from IPF donors or healthy donors. CAFs express higher levels of alpha-smooth muscle actin (a-SMA), HGF, fibroblast specific protein 1 (FSP1), cluster of differentiation 163 . Validated in WB, ICC/IF, IHC-P, IHC-Fr. Supplemental Products. Wounds from IL-6KO, and wild-type mice were collected up to 6 days following wounding. 349 excised breast specimens were immunostained for smooth muscle actin SMA, CD34, FAP-, and Calponin. Alpha smooth muscle actin (-SMA) was recently shown to be present in mouse subcutaneous tissue fibroblasts in the absence of tissue injury. Representative images of stained -SMA fibres in primary human lung fibroblasts following 72 h stimulation with TGF-1 and different concentrations of Compound 408 are shown in Fig. Results: We found that LDs were enriched in lung CAFs and induced the pro-tumoral phenotype of CAFs with increased expression of -smooth muscle actin (-SMA) and Collagen alpha-2 (I) chain (COL1A2). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). The expression levels of fibrotic markers such as -SMA and Collagen I as well as signal transducers such as STAT3, SMAD3, and PPAR were measured by Western blot and quantitative real-time PCR. alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide Formulation Supplied in a saline solution containing BSA and Sodium Azide. Alpha-smooth muscle actin (alpha-SMA) is the actin isoform that predominates within vascular smooth-muscle cells and plays an important role in fibrogenesis. 2010) (Figure 2). CXCR4-positive cells and CD133/VEGFR-2- double positive cells were observed in the stroma surrounding cancer nests, and VEGF was detected in both cancer and stromal cells. Myofibroblasts can contract by using smooth muscle type actin-myosin complex, rich in a form of actin called alpha-smooth muscle actin. Actins are a family of globular multi-functional proteins that form microfilaments.ACTA2 is one of 6 different actin isoforms and is involved in the contractile apparatus of smooth muscle. . Lung fibroblast alphasmooth muscle actin expression and contractile phenotype in bleomycininduced pulmonary fibrosis. FMT protocol has been developed for analysis of transdifferentiation of fibroblasts to myofibroblasts. However, the different sources of TAFs/CAFs, cellular heterogeneity of the tumor microenvironment, similarity of TAFs/CAFs . Activated fibroblasts and myofibroblasts Smooth muscle alpha-actin (SMA) is the definitive marker for the activated cardiac fibroblast population known as the myofibroblast ( Frangogiannis et al., 2000 ), but this actin isoform is also abundant in vascular smooth muscle cells ( Skalli et al., 1989 ). Western blot analysis on rat liver using alpha-SMA antibody ,The lane on the right was blocked with the antigen-specific peptide. alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide . . Myofibroblasts are known to be the primary source of type I and III collagen in fibrotic lesions and this is a consequence of a phenotype differentiation that is . Fibroblasts are the primary cell type of connective tissue and form the extracellular matrix. Furthermore, we explored colocalization analysis . ab5694 reacts with tumors arising from smooth muscles and myoepithelial cells. Synovial expression of fibrotic markers: -SMA, collagen type-I, and PLOD2 in Prg4 gene-trap ( Prg4GT) and recombined Prg4GTR animals were studied at 2 and 9 months of age. 2 B ). In the absence of well-characterized markers, -smooth muscle actin (SMA) is often used to identify pathologic fibroblasts, and some authors have equated SMA (+) cells with contractile myofibroblasts and proposed that these cells are the major source of ECM. Myofibroblasts have the appearance of fibroblasts and neo-express smooth muscle cells' marker alpha-smooth muscle actin (-SMA).6 -SMA is a mechano-sensitive protein that locates in the stress fibers under mechanical load.7 Transforming growth factor-beta1 (TGF-1) can induce fibroblast activation and -SMA expression.8,9 The . Actins are involved in cell motility (alpha, smooth muscle) and the maintenance of the cytoskeleton (beta and gamma, all cells) Antibodies to alpha smooth muscle actin do not detect the other actin isoforms. Local non activated fibroblasts can undergo activation by tumor cells which secrete TGF-beta and SDF-1 (Kojima et al. Other CAF markers previously reported include stromal cell-derived factor-1 (SDF1A), fibroblast activation protein (FAP), and fibroblast-specific protein-1 (FSP-1) [ 28, 32 ]. . Ideal for studying fibroblast-to-myofibroblast differentiation - Includes fibroblast marker Vimentin antibody - Includes myofibroblast marker alpha-SMA antibody - Works on human and mouse samples CAF Marker Antibody Panel (ARG30347) Ideal for studying the heterogeneity of cancer-associated fibroblasts (CAFs) Ferroptosis antibody panel Alpha Smooth Muscle Actin (-SMA) is a general myofibroblast marker, and can be used to identify CAFs. Myoepithelial cells in breast and salivary gland are also stained. Genetic and cell biology studies indicate that tumor growth is determined not only by malignant cancer cells but also by the tumor stroma [].Fibroblasts in the tumor stroma acquire a perpetually activated phenotype and become a subpopulation that can be identified by the expression of -smooth-muscle actin (-SMA) []. Disord. whereas wound closure is significantly inhibited in il-6ko mice, and it has been shown that the expression of -sma, a differentiation marker of the myofibroblast, is induced in pancreatic fibroblast cultures by il-6 treatment ( mews et al., 2002 ), it is unclear what role il-6 plays in the regulation of expression of this contractile protein in Cancer Associated Fibroblasts (CAFs) are thought to regulate tumor growth and metastasis. Actin is a 43000 kDa ubiquitous protein found in all cells. This 1A4 antibody is specific for the alpha form of muscle actin only, which is expressed by smooth muscle cells of blood vessels, myofibroblasts, and myoepithelial cells. Source: Rabbit. [PMC free article] [Google Scholar] 167. 1 Image. Fibroblasts, the most common cells in animal connective tissue, are spindle-shaped cells that produce components of the extracellular matrix (ECM) including collagens and glycosaminoglycans. CAFs express smooth muscle actin alpha (actin alpha ), PDGF . We did not detect any PI16 staining in GLUT-1-positive cells in the inner layer of the perineurium ( Fig. Cultured myofibroblasts are characterized by stress fibers, containing -smooth muscle actin (-SMA) and by supermature focal adhesions (FAs), which are larger than FAs of -SMA-negative fibroblasts. Study material was divided into 5 . DOI: 10.1016/j.bbadis.2016.11.006 Abstract Cardiac myofibroblasts play an important role in myocardial remodeling. Background: Myofibroblasts are cells that have contractile properties and are involved in inflammation, wound healing, fibrosis and oncogenesis in most of the organs and tissues. Seeding density 3,000 cells/well in 96-well plates. Fibroblast Markers : Vimentin antibody: Integrin beta 1 / CD29 antibody : Fibronectin antibody (GTX112794) alpha Smooth Muscle Actin antibody [HL1419 . 3 a. Studies have verified that -SMA+ CAFs could promote tumor progression, confer S5 ). -SMA-positive fibroblasts are called cancer-associated . Significantly higher expression of -SMA was observed in fibroblasts in NPC stroma. Myofibroblasts are metabolically and. The specific markers, such as alpha-Smooth Muscle Actin (-SMA), Vimentin, E-cadherin, -catenin and Smad 2/3 were modulated in myo-hGFs after the induction with TGF-1, at different time points (24, 48 and 72h). The roles of cancer-associated fibroblasts (CAFs) in PDAC have received more attention in recent years. EBM composition and formation are altered during corneal wound healing after injury, infection, and surgery associated with the generation of fibroblasts and myofibroblasts. Background: Cancer-associated fibroblasts (CAFs) comprise a heterogeneous population of stromal cells within the tumour microenvironment. Fibroblast Activating Protein 1 (FAP-1) is a marker for fibroblast activation and by many recognized as the main marker of CAFs. In the absence of well-characterized markers, -smooth muscle actin (SMA) is often used to identify pathologic fibroblasts, and some authors have equated SMA + cells with contractile myofibroblasts and proposed that these cells are the major source of ECM. Myofibroblasts are thus regarded as the key activated fibrogenic cells for normal wound repair and abnormal tissue fibrogenesis, and -SMA is used as a marker for the fibrogenic activity of activated tissue fibrogenic cells [ 26, 34 ]. 275:114061 Huoxin Pill inhibits isoproterenol-induced transdifferentiation and collagen . Full size table. However, the precise fibroblast subsets that drive fibrosis have been poorly understood. Images were digitally analyzed to yield "metrics" related to vasculature and stroma features. Myoepithelial cells in breast and salivary gland are also stained. CAFs exhibit both tumour-promoting and tumour-suppressing functions, making them exciting targets for improving cancer treatments. They are characterized by the expression of the specific fibroblast activation protein-alpha (FAP-), and differ from that of normal fibroblasts exhibiting an immunophenotype of CD34. this study identifies Rac1 as a downstream target for SMA to inhibit smooth muscle cell proliferation and migration. a Endothelial markers CD31, VE-cadherin, and vWF and fibrosis markers alpha-SMA, N-cadherin, vimentin, and collagen I were measured by western blotting in fibroblasts treated with normal cultured EPC-derived exosomes, H/R-induced EPC-derived . The number of myofibroblasts in SSc skin correlates with the modified. Citations (3) FAQs. 1B). SMA. As increasing evidence suggests the heterogeneity of CAFs in PDAC, different CAF subtypes have been shown to support tumor growth, while others . The latter is particularly true for fibroblasts that have differentiated into a myofibroblast phenotype [15,16], i.e. In response to increased mechanical stress caused by increased left ventricular pressure or myocardial stiffening, cardiac fibroblasts differentiate into myofibroblasts characterized by de novo gene expression of actin alpha 2 smooth muscle (Acta2) and assembly of contractile smooth muscle . 5 Intercellular transfer of miR-133 by H/R-induced EPC-derived exosomes inhibits fibroblast MEndoT. The number of cell nuclei was not affected by Compound 408 treatment, even at high concentrations, indicating no impairment of cell viability (Fig. Product: alpha-SMA Antibody. Cancer Associated Fibroblast Marker Antibody Sampler Kit #31549 Reviews () Citations (0) Confocal immunofluorescent analysis of mouse small intestine (left) and skeletal muscle (right) using -Smooth Muscle Actin (D4K9N) XP Rabbit mAb (green). These cells are then capable of speeding wound repair by contracting the edges of the wound. Am J Pathol. CAFs can be identified through a series of markers such as fibroblast-activating protein (FAP), alpha-smooth muscle actin (-SMA), fibroblast specific protein 1 (FSP-1), and vimentin . Rabbit Polyclonal alpha Smooth Muscle Actin antibody. Product Details. Another potential marker for cardiac fibroblast cells is the discoidin domain receptor 2 (DDR2), a collagen receptor that has been used to identify and distinguish cardiac fibroblasts [ 4 ]. 1996; 148:527537. Supplied in a saline solution containing BSA and Sodium Azide. . Careful isolation, identification, and characterisation of CAF heterogeneity is thus necessary for ex vivo validation and . To evaluate whether -smooth muscle actin (-SMA) plays a role in fibroblast contractility, we first compared the contractile activity of rat subcutaneous fibroblasts (SCFs), expressing low levels of -SMA, with that of lung fibroblasts (LFs), expressing high levels of -SMA, with the use of silicone substrates of different stiffness degrees. alpha-SMA was recently shown to be present in mouse subcutaneous tissue fibroblasts in the absence of tissue injury (Storch et al., 2007). (P4H) as marker for activated, collagen-synthesizing fibroblasts, and -smooth muscle actin (SMA) as marker to identify differentiated, mature myofibroblasts. Ca2+- and KCa3.1-dependent processes facilitate "constitutive" alpha smooth muscle actin expression and Smad2/3 signalling in IPF-derived fibroblasts, and thus promote fibroblast to myofibroblast differentiation. A CAF marker antibody cocktail (Table 3) was prepared to detect the 6 chosen CAF markers; fibroblast activation protein alpha (FAP), platelet derived growth factor receptor alpha and beta (PDGFR and PDGFR), and CD26/DPP4 and podoplanin (PDPN), and alpha smooth muscle actin (SMA). 2. Table 1 A summary of current known markers used to determine cardiac fibroblast and myofibroblast cells. Myofibroblasts are the primary mediators of wound closure, and alpha-smooth muscle actin (-SMA) is a marker of fibroblast differentiation to the myofibroblast phenotype. Expression of -SMA mRNA was found to be increased . Trigger 1.25 ng/mL TGF-1. Actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), and myocardium . The aims of the study were to investigate 2 cancer-associated fibroblasts markers, alpha-smooth muscle actin (-SMA), and fibroblast activation protein alpha (FAP) in the stromal microenvironment of benign and malignant ovarian epithelial neoplasms, and to relate their tissue expression with prognostic factors in ovarian cancer. a marker of naive fibroblasts, evidenced a positive expression in time-dependent manner in hGFs stimulated with TGF-1 (10 ng . Tested applications Suitable for: WB, IHC-P more details Species reactivity Application: WB, IHC, IF/ICC, IP, ELISA (peptide) Nishishita R, et al. Catalog # Availability Size / Price Qty; IC1420T-025: IC1420T-100: Add to Cart. We probed for stress fibers and focal adhesions (FAs) in TGF--treated murine fibroblasts and fibroblast migration was quantified rhPRG4. This study aimed to explore the immunohistochemical expression of stemness markers nestin, CD138, and alpha-smooth muscle actin (alpha-SMA) for the characterisation of . In the past, MSC- and fibroblast-derived TAFs/CAFs have been defined by a specific subset of markers, including alpha-SMA, tenascin C, fibroblast-specific protein-1, fibroblast activing protein, and neural-glial antigen . Introduction. Alpha smooth muscle actin antibody (ab5694) stains smooth muscle cells in vessel walls, gut wall, and myometrium. Other markers are: anti-alpha-actin clone 1A4, anti-CD34, anti-HSP47 (Heat Shock Protein 47), anti-SFA (Fibroblast Surface Antigen). Formulation. Methods: The expression of -sma-labeled CAFs in 85 NPCs, 32 nasopharyngitis and 12 metastatic tissues of NPCs was detected by immunohistochemical method. The immunoreactive intensities of SDF-1 and CXCR4 were significantly higher in NPC cells. Fig. Shi, P.; Ge, J.J. miR-21 enhances cardiac fibrotic remodeling and fibroblast proliferation via CADM1/STAT3 pathway. -SMA: a snapshot of CAF heterogeneity As the marker of activated fibroblasts, -SMA, a cytoskeletal protein associated with TGF- production and highly contractile phenotype, is the first identified and most frequent CAF biomarker [ 8, 9 ]. Background. Objective: Evaluation of Myofibroblasts by studying expression of Alpha smooth muscle actin: A marker of Fibrosis, Dysplasia and Carcinoma. Catalog: AF1032. Abstract. While CAFs continue to express a number of common fibroblast biomarkers such as alpha-SMA, FSP1 (S100A4), and FAP, they are often upregulated, whereas PDGFR alpha is reduced. It can arise de novo or from a pre-existing ameloblastoma. However, a recent study showed that -SMA was an inconsistent marker of the fibrogenic function [ 35 ]. Marker expression is quantified using in-house developed algorithms on an HCA platform. The aims of the study were to investigate 2 cancer-associated fibroblasts markers, alpha-smooth muscle actin (-SMA), and fibroblast activation protein alpha (FAP) in the stromal microenvironment of benign and malignant ovarian epithelial neoplasms, and to relate their tissue expression with prognostic factors in ovarian cancer. However, the precise fibroblast subsets that drive fibrosis have been poorly understood. They are involved in healing and granulation tissue formation which occur . Native fibroblasts stain for vimentin and, once activated, stain positive for alpha-smooth muscle actin (SMA) [ 31 ]. As -SMA is the most common marker for fibroblast-to-myofibroblast differentiation, these results suggested that simvastatin and ROCK-inhibitor Y-27632 have an inhibitory effect on the TGF--induced differentiation of orbital fibroblasts into myofibroblasts. There are three main approaches against cancer-associated fibroblasts (CAFs) and their associated molecules for immunotherapy: A Through the immunotherapies or transgenic technologies that targeting CAF markers such as fibroblast activation protein (FAP), alpha-smooth muscle actin (-SMA) and platelet-derived growth factor receptors (PDGFR . alpha smooth muscle actin (-SMA) expressing fibroblasts. Vimentin is the best marker for fibroblast. alpha-SMA could be a useful marker for the detection of early stage fibrosis in liver transplant recipients stopping . A tissue microarray (TMA) with 186 tumors and 91 matched metastases was subjected to immunohistochemistry double staining with endothelial cell marker CD34 and fibroblast and pericyte markers alpha-SMA, PDGF beta R and desmin. ACTA2 (actin alpha 2) is an actin protein with several aliases including alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (-SMA, SMactin, alpha-SM-actin, ASMA). Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Expression of cancer-associated fibroblast markers in advanced colorectal cancer. . Although the morbidity and mortality rates associated with idiopathic pulmonary fibrosis (IPF) are high, there is still lack of powerful and precise therapeutic options for IPF. 2 A and SI Appendix, Fig. Alpha-smooth muscle actin (-SMA) was examined immunohistochemically in intratumoral CAFs of nonmetastatic breast cancers and correlated with clinicopathological data. Through in vitro model, this study sought to determine whether binding of acetylated CCAAT/enhancer binding protein (C/EBP) to alpha-smooth muscle actin (-SMA) promoter could affect the activity of the latter as . Based on these markers, Ohlund and colleagues identified two distinct subtypes of CAFs in human pancreatic cancer: -SMA hi IL-6 low myofibroblastic CAFs and . Alpha-smooth muscle actin (SMA), also known as smooth muscle aortic alpha-actin (ACTA2), is a member of the actin family, a highly conserved group of proteins that play an important role in cell motility, structure and integrity. Development of cancer associated fibroblasts. CAFs have also been shown to originate from and express markers from cells of other origins. Circulating concentrations of fibroblast activation protein alpha in apparently healthy individuals and patients with acute coronary syndrome as assessed by sandwich ELISA. The fibroblast surface markers FAP, antifibroblast, . Label. Ameloblastic carcinoma is a rare malignant odontogenic neoplasm with a poor prognosis. 3 b). It is necessary to. 1. Skin and lung fibrosis in systemic sclerosis (SSc) is driven by myofibroblasts, alpha-smooth muscle actin expressing cells. Double staining for PI16 and the fibroblast markers -SMA (alpha smooth muscle actin) or P4HB (collagen Prolyl 4-hydroxylase) confirmed expression of PI16 in fibroblasts ( Fig. has microinvasion.